| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.238018 |
| Chromosome: | chromosome 3 |
| Location: | 6300309 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193250 | (1 of 1) 2.7.1.160 - 2'-phosphotransferase / Yeast 2'-phosphotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTAACATAGTTGTGCTCGGCGAACCTCC |
| Internal bar code: | CCAATCGCATTTGAATCAGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 99.3105 |
| LEAP-Seq n confirming: | 3889 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCTTCTCAGTACTCGCC |
| Suggested primer 2: | TCAGCTTCGAACCTTGACCT |