| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.238056 |
| Chromosome: | chromosome_4 |
| Location: | 2148366 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g219700 | BBS9 | Bardet-Biedl syndrome-9 associated protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCGTTACCAAATGAGGGTGGGGGAGGGAG |
| Internal bar code: | GATGGTCTGGCGCTTCTAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 423 |
| LEAP-Seq percent confirming: | 97.3061 |
| LEAP-Seq n confirming: | 9572 |
| LEAP-Seq n nonconfirming: | 265 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACCTCAAACCCTTCAA |
| Suggested primer 2: | GGAAGAGGGTGTATGTGCGT |