| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.238064 |
| Chromosome: | chromosome_12 |
| Location: | 1194051 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g489750 | FAP52 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACTCCCGCACCCCGTGCTTGCCCCCACCCC |
| Internal bar code: | GCTCCCGAATTTGCCCAATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 78.5047 |
| LEAP-Seq n confirming: | 168 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGTTTGAACTTGTTCCCG |
| Suggested primer 2: | GACGTAGGCTCAAGGACCAC |