| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238067 |
| Chromosome: | chromosome 7 |
| Location: | 2414034 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328800 | NSG13 | (1 of 8) PF00514 - Armadillo/beta-catenin-like repeat (Arm); Protein expressed during nitrogen-starved gametogenesis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACCGCGGGCTCGTGCACGGTATTCCGCC |
| Internal bar code: | TGCGGACGGACACGTACCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 99.1642 |
| LEAP-Seq n confirming: | 7119 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTCATACATCACGAGTGC |
| Suggested primer 2: | TACTTACAAACCCCCTCCCC |