| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238077 |
| Chromosome: | chromosome 3 |
| Location: | 1593528 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152550 | (1 of 1) IPR000104//IPR001680//IPR011041//IPR015943//IPR017986//IPR020472//IPR027417 - Antifreeze protein, type I // WD40 repeat // Soluble quinoprotein glucose/sorbosone dehydrogenase // WD40/YVTN repeat-like-containing domain // WD40-repeat-containing domain // G-protein beta WD-40 repeat // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAATGGGTGTCAGTGGAGGTGGTGGGTC |
| Internal bar code: | AATTACGTTAGGCCGCAACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 99.8463 |
| LEAP-Seq n confirming: | 3898 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTTGTGCTGTATCAGGT |
| Suggested primer 2: | CAAATCAACGTACCGCACAC |