Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.238085 |
Chromosome: | chromosome 2 |
Location: | 6736303 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g118300 | RHE,HEL12 | (1 of 1) PTHR24031:SF330 - DEAD-BOX ATP-DEPENDENT RNA HELICASE 7; DEAD box ATP-dependent RNA helicase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAAGCCCAATTGCACCAATGCACTTCA |
Internal bar code: | GCGCGATGACAAATCATAAGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 575 |
LEAP-Seq percent confirming: | 98.5646 |
LEAP-Seq n confirming: | 618 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCCCCCATAATACGACCAA |
Suggested primer 2: | AGCGTGTCGATGAGTGTGTC |