Insertion junction: LMJ.RY0402.238097_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.238097
Chromosome:chromosome 13
Location:142466
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g563006 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTACAGCGACCATCGGGATACGGGGCGT
Internal bar code:CTCTTCCTGTCAGGCAGCCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:125
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCCCAAACTAAACCAAA
Suggested primer 2:ACCGGTATTGGTGCTCTCAC