| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.238099 |
| Chromosome: | chromosome 12 |
| Location: | 5170095 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527450 | CAM17 | Calmodulin-like protein; (1 of 1) PTHR10891//PTHR10891:SF599 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAGTACCGGCCCACCAGAAGCTATCTAA |
| Internal bar code: | TGAGTAGCAGCGGGTTGCCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.0719 |
| LEAP-Seq n confirming: | 427 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGATCAAGAAGGTGCG |
| Suggested primer 2: | CGGGTTATGAAGGCACAAGT |