Insertion junction: LMJ.RY0402.238129_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.238129
Chromosome:chromosome_14
Location:2455907
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre14.g624950 DHC10,IDA2,DYH1B Flagellar inner arm dynein 1 heavy chain beta sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GTCTGAGGGTGCGGGCTACCTACCCGCGGC
Internal bar code:GCAACAGCAACAGACGTAGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:639
LEAP-Seq percent confirming:98.1735
LEAP-Seq n confirming:645
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGCTTTCGGGGTATGTGT
Suggested primer 2:GAAGTCAAAGTGCTCCGAGG