Insertion junction: LMJ.RY0402.238133_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.238133
Chromosome:chromosome 15
Location:1203414
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g641266 TPT23,TPT22 (1 of 4) K15285 - solute carrier family 35, member E3 (SLC35E3); Triose phosphate transporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGTCTTGGGCTTATTTCCGTAAACGGCT
Internal bar code:ACCTGCAGTTGAAATAGGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:337
LEAP-Seq percent confirming:99.6672
LEAP-Seq n confirming:1797
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TACACACACACACCCACACG
Suggested primer 2:CGCTCTCCACTTTTCTGGTC