Insertion junction: LMJ.RY0402.238140_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.238140
Chromosome:chromosome 10
Location:6347578
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g465450 (1 of 2) 2.8.2.20 - Protein-tyrosine sulfotransferase / Tyrosylprotein sulfotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGAATTATGGCTTGCGTGTACTCCCCTG
Internal bar code:TACTCGTTCGATACAGGCGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:125
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACGACGGGTTTCACGTACA
Suggested primer 2:TTTGGTTTAGTTTGGGCTGG