| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238201 |
| Chromosome: | chromosome 1 |
| Location: | 5888120 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041950 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTAAGCGCGTCCCTCAAACACCGCTTGA |
| Internal bar code: | AACTCGCGCAAGCGCGTTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 163 |
| LEAP-Seq percent confirming: | 99.1453 |
| LEAP-Seq n confirming: | 348 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGTTTCAGTCCGCGTCT |
| Suggested primer 2: | TTGCTCGTAAAGCTGACCCT |