Insertion junction: LMJ.RY0402.238223_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.238223
Chromosome:chromosome 17
Location:6601350
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g743547 (1 of 16) IPR003439//IPR003593//IPR013525//IPR027417 - ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAACATCTCCGGCGAAGTCACCCCTGGA
Internal bar code:GCAATGAATAGACAGGTAAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:602
LEAP-Seq percent confirming:98.9975
LEAP-Seq n confirming:790
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCTTGTCACTAAAGCACA
Suggested primer 2:TAATCCCCTATCACCCACCA