Insertion junction: LMJ.RY0402.238226_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.238226
Chromosome:chromosome 11
Location:92197
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTGTTGGGATGACGCGACGGCGACAGGC
Internal bar code:TGCACGCCACAGGGAGGGGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:510
LEAP-Seq percent confirming:99.0312
LEAP-Seq n confirming:920
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAAGGTGGTTGCTGTATT
Suggested primer 2:ACAAGTGTGCTGTGTCTGGC