| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238236 |
| Chromosome: | chromosome 14 |
| Location: | 913936 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613652 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGAAACATGAAATGCTGCTGGTCGCGC |
| Internal bar code: | TCACGAAGCGGGTGTAGCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 73.622 |
| LEAP-Seq n confirming: | 374 |
| LEAP-Seq n nonconfirming: | 134 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCCTAAACAAGCGAATC |
| Suggested primer 2: | TATGGGCCACGTAAGGAAAG |