| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238245 |
| Chromosome: | chromosome 9 |
| Location: | 6760962 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409300 | (1 of 5) PF06863 - Protein of unknown function (DUF1254) (DUF1254) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAATGGGTGAGGAAGATTGGGTGTTGGG |
| Internal bar code: | TCTTGTCGTCGCCACGCTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 99.4027 |
| LEAP-Seq n confirming: | 2330 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTAGCGGCACCTAACGA |
| Suggested primer 2: | GAAAGGAAAGCACAGAACGC |