| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.238270 |
| Chromosome: | chromosome 17 |
| Location: | 3495881 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725100 | (1 of 1) PTHR11390:SF20 - DNA TOPOISOMERASE 3-BETA-1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAGCAGCCTACACGATTTCAACTCCCCA |
| Internal bar code: | CGCATTTGACGAACGTCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 617 |
| LEAP-Seq percent confirming: | 99.8011 |
| LEAP-Seq n confirming: | 1505 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGACGTGCCCAATGACCT |
| Suggested primer 2: | GGCTCGATCTATGCAAGAGG |