| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238301 |
| Chromosome: | chromosome 16 |
| Location: | 5655317 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679400 | (1 of 1) PF16501 - S phase cyclin A-associated protein in the endoplasmic reticulum (SCAPER_N) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGCATGCTGCTGAGATGGTTGTATTG |
| Internal bar code: | GGGAGGCACATGAGCTCATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 99.1071 |
| LEAP-Seq n confirming: | 333 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTGACCTGGGTGAGATG |
| Suggested primer 2: | GGAAGGAGGACCCAGCTATC |