Insertion junction: LMJ.RY0402.238321_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.238321
Chromosome:chromosome 9
Location:1657410
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g397050 (1 of 28) PTHR31600:SF2 - TINY MACROCYSTS PROTEIN B-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTTATGCATGCAGGGCCGCCTGCTCCAC
Internal bar code:AGCGTCGCCCCACATTAGATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:646
LEAP-Seq percent confirming:94.3262
LEAP-Seq n confirming:133
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCAGGGTCAGCTAGGTTC
Suggested primer 2:CAGATGGACTTCGGACCTGT