| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238323 |
| Chromosome: | chromosome 16 |
| Location: | 658580 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691750 | AMI1 | putative formamidase/acetamidase; (1 of 1) 3.5.1.49 - Formamidase / Formamide amidohydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACCGCCGCCGTTGTCGCGGTCAAACGTG |
| Internal bar code: | CTGGGTGCGCTCCTCGGTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 99.2958 |
| LEAP-Seq n confirming: | 846 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGTGTTAAAGAGCCGGG |
| Suggested primer 2: | GCGTCTGTGTCAAAGCAAAA |