Insertion junction: LMJ.RY0402.238356_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.238356
Chromosome:chromosome 16
Location:1303006
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g651500 (1 of 1) K12180 - COP9 signalosome complex subunit 7 (COPS7, CSN7) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAAACGTCCCCTCCCCCGCGCTCCCGAA
Internal bar code:GGGGACTCTCGGTCACGTCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:172
LEAP-Seq percent confirming:80.5195
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCGAGTAGCGTTGTCGTC
Suggested primer 2:TAATGCCTCTCACACCTCCC