| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.238365 |
| Chromosome: | chromosome 10 |
| Location: | 1520324 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428966 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTCAGCAACATTCACTCAGATTAAGTG |
| Internal bar code: | GTCATGTCGCGGATTGAGATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 668 |
| LEAP-Seq percent confirming: | 8.67347 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 179 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAAAGGTCTTCAGGGGG |
| Suggested primer 2: | GCCAAACCTGCTACCTTCTG |