| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.238413 |
| Chromosome: | chromosome 1 |
| Location: | 2008560 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010832 | (1 of 1) PTHR12411:SF389 - CATHEPSIN L1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTAAACCCGCCTTCACTTCTTAAACAA |
| Internal bar code: | GTAGATCGATGATTTGTTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 99.0788 |
| LEAP-Seq n confirming: | 968 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGCTCAGGTAGAAGGAG |
| Suggested primer 2: | GACGGGATACGACACGAGTT |