Insertion junction: LMJ.RY0402.238419_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.238419
Chromosome:chromosome 2
Location:1025700
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g080500 (1 of 5) 2.4.1.17 - Glucuronosyltransferase / Uridine diphosphoglucuronyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGGGAAAAGCGGGTCGGGCACGAGGACG
Internal bar code:AAGCGACATGCAAGGTAGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:456
LEAP-Seq percent confirming:99.2798
LEAP-Seq n confirming:965
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ACATATGGCCCCAAACACAT
Suggested primer 2:GCTTACGCATTCCTACCTCG