| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238421 |
| Chromosome: | chromosome 11 |
| Location: | 2386337 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g474750 | (1 of 1) 2.4.2.39 - Xyloglucan 6-xylosyltransferase / Xyloglucan 6-alpha-D-xylosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACATTAAAGGACACGGTACTTTTGGAG |
| Internal bar code: | GTCGTGAAATGGACCCTCCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 98.4429 |
| LEAP-Seq n confirming: | 569 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTAGGCAGCAGATGGGTA |
| Suggested primer 2: | ACACACACACACACACACCG |