Insertion junction: LMJ.RY0402.238421_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.238421
Chromosome:chromosome 14
Location:1505151
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre14.g618400 ABCA3 (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase; ABC transporter 3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCAAGCTCAAGCTGGACTTTTCAAACCT
Internal bar code:ACGGGCGTATCTATAACGGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:394
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGTACTTGCCGCTGAAGTTG
Suggested primer 2:ACACCCCCATGTAATGTCGT