| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238423 |
| Chromosome: | chromosome 14 |
| Location: | 1358310 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617150 | RLS4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTTCCACCACGGAACGGTTGACCACTC |
| Internal bar code: | GCCCTCAGTCCTTAGGTCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 98.5646 |
| LEAP-Seq n confirming: | 206 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCGTTTTGACTGGTGTT |
| Suggested primer 2: | GTAGCAGTTAGACCGCCTCG |