| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238426 |
| Chromosome: | chromosome 3 |
| Location: | 1930242 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155350 | PPO4 | Pyridoxamine 5'-phosphate oxidase-like protein; (1 of 1) 1.14.13.142 - 3-ketosteroid 9-alpha-monooxygenase / 3-ketosteroid 9-alpha-hydroxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAGGAGGCAGCGGTGTCGGCGACGGAG |
| Internal bar code: | ACGCTGGTGTAATTCTCAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 184 |
| LEAP-Seq percent confirming: | 98.7097 |
| LEAP-Seq n confirming: | 153 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCCTACACACCCTTCCA |
| Suggested primer 2: | GATTAGGAGCGGACATCCAA |