| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238432 |
| Chromosome: | chromosome 15 |
| Location: | 1295721 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641750 | (1 of 3) PF05495 - CHY zinc finger (zf-CHY) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGTGTATCTATAGGTGATTGGACATAA |
| Internal bar code: | ATCGGACCCCGGCGGCCGGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 96.648 |
| LEAP-Seq n confirming: | 519 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGAACTGTAGGTCGTCG |
| Suggested primer 2: | TTTTGCTAGCCACGAGGTTT |