| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.238439 |
| Chromosome: | chromosome 3 |
| Location: | 7923480 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202250 | (1 of 1) PTHR17598:SF13 - DNA POLYMERASE DELTA SUBUNIT 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCCCGCCGGTTTGGCCCCTTGTCGTCT |
| Internal bar code: | AGACGTACGTCCGCCCGGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 334 |
| LEAP-Seq percent confirming: | 98.3287 |
| LEAP-Seq n confirming: | 353 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGTAATCAAGGTGGCCG |
| Suggested primer 2: | GCCACAGACGACTCATGCTA |