| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.238448 |
| Chromosome: | chromosome 3 |
| Location: | 5642160 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187350 | TUD1,UNI3 | (1 of 1) K10390 - tubulin delta (TUBD); Delta tubulin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAACCCTGCCACGTCTGCGAGCAATAG |
| Internal bar code: | TCATTGCACTCAAGCTTGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 172 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCACGTTAACTGAGACCC |
| Suggested primer 2: | CTCCTACCTGCGGACTGAAG |