Insertion junction: LMJ.RY0402.238449_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.238449
Chromosome:chromosome 4
Location:3861402
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g230242 MOT40 (1 of 1) IPR007914//IPR015422 - Uncharacterised protein family UPF0193 // Pyridoxal phosphate-dependent transferase, major region, subdomain 2; Predicted protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATGGTCTTTGGGCTGTCGGACATGTCAC
Internal bar code:CTGTTCCGTTTGTCTGGCAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:190
LEAP-Seq percent confirming:98.8889
LEAP-Seq n confirming:89
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCTCAAAACCCTCAGAC
Suggested primer 2:CCAGACCTATTCGGTGCAAT