| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238462 |
| Chromosome: | chromosome 2 |
| Location: | 2736330 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g093750 | NRX2 | Nucleoredoxin 2; (1 of 3) K17609 - nucleoredoxin [EC:1.8.1.8] (NXN) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGAGCTCCCCTCTCGCCAAACCGCTGC |
| Internal bar code: | GGCGAGTTGGACATCCCCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 210 |
| LEAP-Seq percent confirming: | 99.336 |
| LEAP-Seq n confirming: | 4039 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACACCACCTCCCAGTCAG |
| Suggested primer 2: | GACATTCTTGGGTGCAGGAT |