| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238474 |
| Chromosome: | chromosome 16 |
| Location: | 1982835 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656600 | TRX19 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGCCACTTGCTACTCTTATGCTGAGCT |
| Internal bar code: | GATGAGGGTAGCGATAGCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 98.4211 |
| LEAP-Seq n confirming: | 374 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGAAAACAGAAAGCCGAC |
| Suggested primer 2: | GAAATCAGGTTGCCAATGCT |