| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238477 |
| Chromosome: | chromosome_12 |
| Location: | 8001809 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g552006 | antisense | 5'UTR|5'UTR_intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGGCGGTGTGGAGGGACAAGTACTCAGGC |
| Internal bar code: | AGCCGCACTGTGCGTAGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 99.3651 |
| LEAP-Seq n confirming: | 1565 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCCTCTCCCTGTCATTA |
| Suggested primer 2: | ATGGCGAGAAGATGAGCACT |