Insertion junction: LMJ.RY0402.238479_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.238479
Chromosome:chromosome 7
Location:5480844
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g350867 (1 of 37) PF13639 - Ring finger domain (zf-RING_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGCAGGATCTGCCACTTGTGCGACTGC
Internal bar code:GGGTGATTGTTTCACCGGCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:205
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCCACAACATGATCAGC
Suggested primer 2:TACTCCGATGGATACTGGGG