| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.238488 |
| Chromosome: | chromosome 16 |
| Location: | 557943 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692500 | (1 of 19) PTHR31251:SF1 - SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCTTGTTGGCGGCCTGAACGGCGGCC |
| Internal bar code: | AGGGTACGTGCTGTTTCGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 98.3933 |
| LEAP-Seq n confirming: | 1531 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCTAATGGTGGTGCTGCC |
| Suggested primer 2: | GACACGAAGCTGTCTCCTCC |