| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238519 |
| Chromosome: | chromosome 12 |
| Location: | 5833965 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533550 | PYK1 | (1 of 1) PTHR11817:SF5 - PYRUVATE KINASE-RELATED; Pyruvate kinase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTTGGCTCCCGAACGACCATAATCACC |
| Internal bar code: | TGGGCGTCATGGCACCTTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 97.4619 |
| LEAP-Seq n confirming: | 192 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCGGGTCTGATAACACT |
| Suggested primer 2: | GAGTGCACCCATGTGATTTG |