| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.238525 |
| Chromosome: | chromosome 17 |
| Location: | 3448138 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724650 | (1 of 9) PTHR13789//PTHR13789:SF186 - MONOOXYGENASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGAGCAGCCAGGCCAGCCCCTATACCGT |
| Internal bar code: | CTGAGGCTGGGGACGCCCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 99.823 |
| LEAP-Seq n confirming: | 564 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTTAAGGGCTTTCGGTGA |
| Suggested primer 2: | GGCTGGAAATTTGTTCGTGT |