| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238575 |
| Chromosome: | chromosome 12 |
| Location: | 3714483 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514700 | PPHC1,PPH | Pheophytinase 1, chloroplastic; (1 of 1) PTHR10992:SF879 - PHEOPHYTINASE, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCACCGCCCTCCGTTGCCTGTAACCCTC |
| Internal bar code: | CGTGACTTTTCTCGATGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 108 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 107 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGAACGCACTCTACCC |
| Suggested primer 2: | GTGTAGATCCATGCGGTGTG |