| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.238632 |
| Chromosome: | chromosome 12 |
| Location: | 6440415 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537900 | (1 of 1) PF11927 - Protein of unknown function (DUF3445) (DUF3445) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCCAATGGGCATGCCCGACCGGCCCGAA |
| Internal bar code: | AGATGACAGCGAGGAGATCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 90.0114 |
| LEAP-Seq n confirming: | 3172 |
| LEAP-Seq n nonconfirming: | 352 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCGTGCCAAGTTTTGTT |
| Suggested primer 2: | GCCTAGCGGTGCTTACAAAC |