| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238656 |
| Chromosome: | chromosome 14 |
| Location: | 4143884 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g634236 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCACACCCCCGACCACACACCCCCGACC |
| Internal bar code: | GCTCGATGTCCCACATGCAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 31 |
| LEAP-Seq percent confirming: | 95.7447 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATCTTAAGGCAGTGGCG |
| Suggested primer 2: | TTCACGCCTACCCCAGATAC |