| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238691 |
| Chromosome: | chromosome 12 |
| Location: | 6541065 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g539050 | (1 of 1) IPR000363//IPR016159 - Alpha 1D adrenoceptor // Cullin repeat-like-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGCGCCCACGCGACTTGCTGCGCGTGC |
| Internal bar code: | AGGCTGCACGGAAGCGCCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 215 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGGCTTCTTGACAGGAG |
| Suggested primer 2: | TAGAGCAGCACTCGTCAGGA |