Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.238700 |
Chromosome: | chromosome 10 |
Location: | 5344379 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g458050 | BCA3 | Branched chain amino acid aminotransferase; (1 of 1) 2.6.1.21//2.6.1.42 - D-amino-acid transaminase / D-aspartic aminotransferase // Branched-chain-amino-acid transaminase / Transaminase B | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCTGTCACTGGACGGTGGGGCACGGAG |
Internal bar code: | GGCGTTCTCACCCCCCCTATCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 865 |
LEAP-Seq percent confirming: | 99.3265 |
LEAP-Seq n confirming: | 3097 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTACATAACCATGGCCCACC |
Suggested primer 2: | CTCTATCTAGCGCCGTGTCC |