| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.238705 |
| Chromosome: | chromosome 10 |
| Location: | 6521823 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466800 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATTATATTGACAGCATGAAACAATATA |
| Internal bar code: | GTAGATCGGGGAGAAGCGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 160 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTTGGACCACTCCACTT |
| Suggested primer 2: | CTTGCCTAATCACACGCAGA |