Insertion junction: LMJ.RY0402.238717_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.238717
Chromosome:chromosome 17
Location:4196255
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g730150 (1 of 26) IPR029058//IPR029059 - Alpha/Beta hydrolase fold // Alpha/beta hydrolase fold-5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTAGAATGCACAGACCACAGCTGAAAGC
Internal bar code:CAATACGTGTTCGATCTGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:153
LEAP-Seq percent confirming:68.9655
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTAAGCGGGGACGACATAGA
Suggested primer 2:GCGCTGGTCACGGTACTACT