| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238728 |
| Chromosome: | chromosome 12 |
| Location: | 5487255 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530900 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCCTGGTGCGTGCGCGTGGTGTGTGGG |
| Internal bar code: | GGGAGTAACCACCCGCGCTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 451 |
| LEAP-Seq percent confirming: | 80.1136 |
| LEAP-Seq n confirming: | 423 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCTTGCTTGAGGGTTTC |
| Suggested primer 2: | CTGGTTGATGACGCAGAAGA |