Insertion junction: LMJ.RY0402.238742_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.238742
Chromosome:chromosome 11
Location:1102236
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467678 COP6,HKR2 (1 of 5) IPR001660//IPR013761 - Sterile alpha motif domain // Sterile alpha motif/pointed domain; Histidine cyclase rhodopsin 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCAACCCCTCCGTGGCCCCTTCTCCTCC
Internal bar code:AAGGATATTAAAGGACGACCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:641
LEAP-Seq percent confirming:95.0739
LEAP-Seq n confirming:193
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGTCAGGTTGTACAGGAT
Suggested primer 2:AGAGCGAGGAAGGGAAGAAG