| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238760 |
| Chromosome: | chromosome 16 |
| Location: | 4898254 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAGTTACGTGTACGGGGTGAGCACATG |
| Internal bar code: | GGGGCTATCGGGGGTCAGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 96.0396 |
| LEAP-Seq n confirming: | 291 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACACTAAGCCGTGCCTG |
| Suggested primer 2: | GCTCTGACCTTCTCCACAGC |