| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238770 |
| Chromosome: | chromosome 2 |
| Location: | 2448674 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091700 | RIB72 | Flagellar protofilament ribbon protein 72; (1 of 1) PF06565//PF13499 - Repeat of unknown function (DUF1126) (DUF1126) // EF-hand domain pair (EF-hand_7) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGTGTTGCAGCTGGGTCCCCCTTAGTC |
| Internal bar code: | GAGGTAGACCCTGATTTTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 98.9691 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTAAGCAGGGAGAGGCAC |
| Suggested primer 2: | GTTCGAGGGTGTCGTTCATT |